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Effect of deoxynivalenol and T-2 toxin in malt amylase activity BABT
Garda-Buffon,Jaqueline; Baraj,Edlira; Badiale-Furlong,Eliana.
The objective of this work was to evaluate the effect of DON and T-2 toxin levels on the malt amylase activity. The malt was contaminated in accordance with central composite design experiment with DON and T-2 toxin levels until 1000 ng/g. The activities of the enzymes were evaluated by Bernfeld method. The increase in T-2 toxin concentration inhibited the α-amylase activity. However, the increase of both toxins concentration caused inverse effect. The interaction between toxins indicated synergetic effect on the β-amylase activity. An increased activity occurred when the toxins contamination levels in malt were higher (1000ng/g malt). The trichothecenes interfered with the performance of aminolitic enzymes in the stage of malting, resulting in a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aminolitic enzymes; Fermentation; Trichothecenes; Barley.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300002
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Evaluation of the supplementation of a feed additive as a potential protector against the adverse effects of 2.5 ppm T-2 toxin on growing broiler chickens Arq. Bras. Med. Vet. Zootec.
Diaz,G.J.; Vargas,M.L.; Cortés,A..
ABSTRACT A trial was conducted to evaluate a feed additive containing epoxidase activity from a bacterium (Mycofix-S) as a potential protection against the adverse effects of 2.5 ppm dietary T-2 toxin in male growing broiler chickens. A total of 144 one-day-old Ross 308 male chicks were individually wing-banded and allotted into each of the four experimental groups. Group 1: negative control, no T-2 toxin or additive; group 2: Mycofix-S, 2.5 g/kg; group 3: positive control, 2.5 ppm T-2 toxin; group 4: 2.5 ppm T-2 toxin + 2.5 g/kg Mycofix-S. Feed and water were provided ad libitum for 28 days (days 1 to 28 of age). Each experimental treatment was replicated 6 times, with 6 birds per replicate pen. Response variables included performance parameters, serum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ALP; Amylase; Epoxidase; Mycotoxin decontamination; Trichothecenes.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000300709
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Profile of the alcohols produced in fermentations with malt contaminated with trichothecenes BABT
Reinehr,Christian Oliveira; Furlong,Eliana Badiale.
In order to study the influence of mycotoxins on the production of alcohols, a fermentative process on a laboratorial scale was simulated. Malt was contaminated with deoxynivalenol and T-2 in different ratios (up to 500 ppb), according to a 3² factorial design, and the alcohols obtained after the fermentation were determined through gas chromatography. The results showed that trichothecenes influenced the profile of the alcohols produced by Saccharomyces cerevisiae during the fermentative process of malt, especially the profile of methyl and isoamyl alcohols.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Deoxynivalenol; Fermentation; Malt; T-2 toxin; Trichothecenes.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400013
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Survey of mycotoxins in Southern Brazilian wheat and evaluation of immunoassay methods Scientia Agricola
Duffeck,Maíra Rodrigues; Tibola,Casiane Salete; Guarienti,Eliana Maria; Del Ponte,Emerson Medeiros.
ABSTRACT One hundred commercial wheat grain samples were collected during the 2015 sea-son across 78 municipalities in the states of Paraná (PR), Rio Grande do Sul (RS), and São Paulo (SP), Brazil. Separate subsamples were analyzed for the concentration of deoxynivalenol (DON), zearalenona (ZEA) and ochratoxin A (OTA) mycotoxins using two methods: UHPLC-MS/MS (reference method) and a commercial enzyme-linked immunosorbent assay (ELISA) (AgraQuant®). The OTA mycotoxin was not found in the samples by both methods. DON and ZEA were detected in 55 % and 39 % of the samples by the reference method, with overall mean levels of 795.2 μg kg−1 and 79.78 μg kg−1, respectively. There was a significant and positive correlation (Spearman rank) between DON and ZEA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; UHPLC; ELISA; Trichothecenes; Ochratoxin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000400343
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Toxigenic potential of Fusarium graminearum isolated from maize of northwest Argentina BJM
Sampietro,D.A.; Apud,G.R.; Belizán,M.M.E.; Vattuone,M.A.; Catalán,C.A.N..
Twenty six isolates of Fusarium graminearum from grains of maize hybrids harvested in ±west Argentina were grown on autoclaved rice grain to assess their ability to produce type B trichothecenes. Chemical analysis indicated that 38% of isolates were nivalenol (NIV) producers only, 31% were major NIV producers with high DON(deoxynivalenol)/NIV ratios, 8% were major DON producers with minor NIV production, and 23% were DON producers only. Isolates showed a high variability in their toxigenic potential which was not related to fungal biomass. The distribution of the different chemotypes as well as the high and the low trichothecene-producing Fusarium isolates could not be associated to a geographical origin. Our results confirmed for the first time that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium graminearum; Trichothecenes; Maize; Chemotypes.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200011
Registros recuperados: 5
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